BOUND Antibody recognized Anti-Idiotypic Antibody Discovery Service

Introduction Drug-target Complex Anti-idiotypic Antibodies Published Data

Creative Biolabs provides anti-idiotypic antibodies detecting bound antibodies. We utilize competitive selection of immunized phage display library to isolate anti-idiotypic antibodies detecting bound antibodies exclusively: antibody-antigen complex is immobilized as selection target, competing selection with human serum, free antigen, free antibody and free control antibody.

Introduction

Anti-idiotypic antibodies are key reagents for PK studies of antibody drugs. Different assay formats designed with different types of anti-idiotypic antibodies can be used to specifically detect various forms of antibody drugs, i.e. free, bound or total amount of antibody drugs.

Drug-targetComplex Anti-idiotypicAntibodies

According to the different binding modes, anti-idiotypic antibodies can be divided into antigen-blocking type, antigen-non-blocking type and drug-target complex type.

Fig. 1 Anti-idiotypic antibody types. (Creative Biolabs Original) Fig. 1 Types of anti-idiotypic antibody.

Drug target complex anti-idiotypic antibodies, this type of antibody can specifically recognize the drug target complex and can be used to detect bound antibody drugs.

The guided selection method allows isolation of rare specificities, such as drug target complex binders that can be used to quantify bound drug, as opposed to free drug levels. These complex-specific antibodies bind neither the antibody drug nor the drug target when on their own, and thus can be used to detect bound therapeutic antibodies directly. This sensitive tool allows the developer to design superior assays for analysis of levels of bound drug, as opposed to measure free drug only. More sensitive and more robust assays that avoid the bridging format can be built, leading to improved assays for pharmacokinetic studies.

Anti-ID antibodies’ properties:
• Antibody-antigen complex specific
• Not inhibitory
• Detects bound antibody exclusively

Detection mechanism of anti-idiotypic antibody binding to BOUND antibody. (Creative Biolabs Original) Fig.2 Binding mode of anti-idiotypic antibody detecting BOUND antibody.

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Published Data

Anti-IDs epitope type characterization in project E. Fig.3 SPR analysis of epitope type determination.¹

Researchers have developed an effective platform for producing anti-IDs essential for bioanalytical pre-clinical and clinical assessments of therapeutic antibodies. Utilizing rabbit single B cell sorting and cloning post-immunization with therapeutic drug Fab fragments, the team isolates CDR-specific B cells through a selective gating process to filter out non-specific cells. Enzyme-linked immunosorbent assay screens cultures of these cells, targeting drug-binding capabilities, and selects positive B cell lysates for IgG variable region cloning. This innovative approach yields high-affinity, broad-epitope anti-IDs, supporting pharmacokinetic and immunogenicity analyses within 12 weeks, successfully developing assays across 11 therapeutic antibodies.

Creative Biolabs provides you with high-level anti-idiotypic antibody production services for detecting BOUND antibodies. We have first-class technology and rich experience to help you develop antibody drugs.

Reference

Lin, WeiYu, et al. "Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology." Plos One 15.12 (2020): e0244158. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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