Membrane Protein Antigen Preparation Services for Antibody Discovery

Empowered by plenty of cutting-edge platforms and technologies, Creative Biolabs has long been committed to high-quality membrane protein antibody discovery services. With more than 10 years' experience, we are dedicated to helping our clients prepare membrane proteins as antigens in various formats for antibody generation & selection.

Since their vital physiological functions, membrane proteins are popular drug targets for therapeutic and diagnostic applications. But the intricate structures and low natural abundance make most membrane proteins very difficult to express and isolate. How to obtain membrane proteins in their stable, native and functional format poses a major challenge for most relevant research programs. In particular, discovery of anti-membrane antibodies is often lagged due to the difficult antigen preparation process. In aim of overcoming this issue, Creative Biolabs has established a versatile membrane protein technique platform providing multiple strategies.

Specific Membrane Protein Antigen Preparation

With the help of our cutting-edge platform, Creative Biolabs has successfully obtained many functional immunogens using multiple approaches. They can serve as excellent antigens for raising high specific, high-affinity antibodies by means of phage display, hybridomas, B cell sorting technology.

Membrane Protein Antigen Formats in Creative Biolabs

DNA Immunization: With the help of DNA immunization, the DNA of target protein can be directly introduced to host cells, resulting in spontaneous protein expression in native structures and native membrane environments. Meanwhile, it has minimal cytotoxicity to a wide range of hosts. It's also very simple-to-operate, so different immunogen designs can be easily tested.
Recombinant Proteins Immunization: Based on our world-leading cell-based expression and cell-free expression systems, Creative Biolabs offers multiple membrane protein formats, including: detergents, nanodiscs, liposome, polymers and MP-VLPs. Well-designed peptides can also be utilized as surrogate of full-length membrane proteins with similar immunogenicity and characteristics but with higher stability and solubility.
Whole Cell Antigen Presentation Immunization: Creative Biolabs is proficient in constructing stable cell lines with membrane protein overexpressing from CHO, HEK, dendritic cells, etc. With years of efforts, we have tailored over 200 GPCR/ion channel stable cell lines with high expression levels. They can be employed directly for membrane protein isolation or host immunization.
Exosomes Immunization: The orientation of membrane proteins on exosomes is close to the orientation of the whole cell. Besides, exosomes can be acquired from numerous cell types and have the similar ability with their source cells. They have stable storage property and can cause strong immune response without the additional adjuvant.

Membrane protein antigen formats (Creative Biolabs original)

We are more than happy to share our experience and help our customers to solve all kinds of challenges in this regard. Please feel free to contact us for more details.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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