Creative Biolabs has specialized in supporting antibody and immunoassay development programs with the highest demands. We have experienced experts and advanced platforms that enable us to provide unparalleled contract immunoassay development services targeting a panel of biomarkers. Here, we introduce our IVD immunoassay development services for BP230 autoantibody.
Introduction to BP230
BP230, also known as bullous pemphigoid antigen 1 or BPAG1, belongs to the plakin protein family. BP230 plays an important role in the formation of the cytoskeleton. It has been regarded as a cytolinker to connect the members of the cell cytoskeleton at the plasma membrane. BP230 can be expressed in a number of tissues, including heart and skin, and studies have demonstrated that BP230 can maintain the integrity of these tissues under mechanical pressure. Meanwhile, it is an important component of semi-bridged and extracellular matrix connective epithelial cells. The genetic variants of BP230 can lead to a wide variety of skin disorders, such as paraneoplastic pemphigus and bullous pemphigoid (BP). In addition, BPAG1 has four isoforms, BPAG1-a, BPAG1-b, BPAG1-n, and BPAG1-e. Besides, BPAG1-a-deficient mice have been established and studies suggested that the absence of this gene may be the cause of neurodegeneration.
Fig.1 BP230 and plectin bind to keratin intermediate filaments.1
Autoantibodies Targeting BP230 for BP Diagnosis
BP is an autoimmune blistering disorder of the skin that usually affects the elderly. The disease is associated with tissue-bound and circulating autoantibodies directed against components of the epidermal basement membrane zone. One of the autoantigens that autoantibodies recognize is BP230. Studies have reported that the anti-BP230 antibody is a useful serologic marker of the disease and co-relates well with the disease activity. Commercial ELISA kits using recombinant BP230 proteins as the antigenic substrates provides an easy and rapid approach for detecting anti-BP230 antibodies in BP sera.
IVD Immunoassay Development Services Provided by Creative Biolabs
Immunoassays, such as ELISA, are based on the specific recognition between the analytes and the pre-coated antigen or antibodies. Development of immunoassays can be difficult, with challenges including establishing method selectivity, specificity and range of quantitation as a result of nonspecific background signal, matrix interference, lack of linearity and antibody interference. With extensive experience in immunoassay development, Creative Biolabs offers services including but not limited to recombinant protein expression, antibody development, protocol establishment, assay validation, and manufacturing. Our professional project management team assists you throughout the entire course of a project. Creative Biolabs offers comprehensive contract development services including:
High-quality antigens are of great importance for ensuring the sensitivity and specificity of diagnostic kits to detect autoantibodies in patient serum. Creative Biolabs is equipped with expertise in the production and purification of autoimmune antigens from native sources.
Our approach is to listen to your exact needs and discuss with you to design a mile-stoned work package to deliver on your exact requirements for your IVD immunoassay development program. Please feel free to contact us for more information and discuss your project needs.
Reference
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