As a preeminent contract antibody development company, Creative Biolabs offers high-quality in vitro diagnostic (IVD) antibody development services targeting a wide range of biomarkers. Here, we focus on the IL-10 marker of sepsis. We are committed to providing the highest quality of custom services and products at the most reasonable prices.
Interleukin 10 (IL-10)
Interleukin 10 (IL-10), alternatively known as human cytokine synthesis inhibitory factor (CSIF), is a cytokine with anti-inflammatory, which is encoded by the IL10 gene in humans. It is primarily secreted by monocytes, and to a lesser degree by lymphocytes, namely mast cells, type 2 T helper cells (Th2), CD4+ CD25+ Foxp3+ regulatory T cells, as well as in a certain subset of activated T and B cells. IL-10 has an essential role in infection via limiting the immune response to pathogens and thus protecting the host from damage. During an infectious attack, IL10 could suppress the activity of cytokines, MHC class II Ags, and costimulatory molecules on macrophages, all of which are responsible for optimal pathogen clearance but also involved in tissue damage. It also promotes B cell survival, proliferation, and antibody production. Accordingly, IL-10 can both impede pathogen clearance and ameliorate immunopathology.
Fig.1 The sources and targets of IL-10 during infection. (Couper K N, et al. 2008)
The Role of IL-10 in Sepsis
It is fully recognized that the production of inflammatory cytokines, such as IL-1 and TNF-α, plays a vital role in the pathogenesis of sepsis. Currently, there is growing recognition that IL-10 secretion in sepsis is a direct result of a more proximal proinflammatory cytokine production, particularly TNF-α. Subsequently, the endogenous production of IL-10 functions in an allosteric-like manner to inhibit endogenous TNF-α production. IL-10 circulates in the blood of patients with sepsis syndromes, and it has been shown that increased concentrations of IL-10 in the blood and tissue compartments are always indicative of the magnitude of the inflammatory stress, which is correlated with an adverse clinical outcome.
Fig.2 IL-10 signaling pathway. (Berti FCB, et al. 2017)
Antibodies are core elements for antibody-based immunoassays for detecting and quantifying antigens of interest in all kinds of samples such as the serum, urine, and tissues. IVD antibodies are extensively used for disease screening, prognosis, and therapeutic monitoring. With our versatile IVD platform, Creative Biolabs is proud to develop novel anti-IL-10 antibody from scratch to commercial IVD kit (we can also start with provided antibody candidates).
References
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