Post-drug exposure effects are important to understand and optimize drug efficacy. Creative Biolabs has developed in vitro models to help you study of post antifungal effects (PAFEs).
The post antifungal effect (PAFE) refers to growth suppression of surviving fungi following the removal of antifungals in the absence of host defense mechanisms. It is a phenomenon which is observed both in vitro and in vivo. Antifungals are capable of exerting many different effects on surviving fungi even though they are just transitorily exposed to the drug. The mechanisms of post antifungal effect include delayed recovery of enzyme and non-enzyme protein activities; changes in cell morphology, metabolism, growth and generation time; changes in cell receptors; susceptibility to phagocytosis; and altered susceptibility to an antifungal following re-exposure. PAFE is more enduring comparing the effect of changes in growth rate.
The ability of an antifungal to induce a PAFE is an attractive property since its ability to suppress fungal growth even through concentrations fall below the MIC. The knowledge of PAFE would be clinically useful in determining dosage regimens of a new drug. For oral fungal diseases, like oral candidosis, the diluent effect of saliva and the cleansing effect of the oral musculature tend to reduce the effective therapeutic concentrations and thus influence the bioavailability of antifungal agents. Evaluating PAFE for the oral fungal disease is more important than evaluating antifungal susceptibility only.
Creative Biolabs offers customized PAFE assay for both yeast and filamentous fungi (spores). The steps of PAFE assay are:
Creative Biolabs can make a tailored optimum PAFE system according to your needs. For more detailed information, please feel free to contact us or directly sent us an inquiry.
For Research Use Only.