Custom Polymers Service

Double-stranded RNA (dsRNA) gene expression silencing is a revolutionary research achievement in the field of biotechnology in the late last century. This phenomenon, called RNA interference (RNAi), provides us with a way to fight many diseases that are difficult to cure with conventional therapies, thereby greatly enriching the technical means of gene therapy. Polymer-mediated siRNA delivery has several advantages, such as easy chemical modification, good biocompatibility, versatility, and fusion with inorganic materials, which can solve various obstacles related to effective siRNA delivery. In order to obtain good clinical treatment results, Creative Biolabs can customize polymer delivery systems for customers, and we have the following polymer-carriers for you:

• Polyethyleneimine (PEI)

Constructing a cationic carrier with PEI can not only solve the problem of cytotoxicity, but also provide sufficient protection against serum or RNase through effective complexing with siRNA. In addition, PEI can exert a "proton sponge effect" to induce the release of siRNA into the cytoplasm. Incorporating low molecular weight PEI into other polymer structures can also optimize the target specificity of the carrier, circulation cycle, serum stability and other parameters.

• Poly(dl-lactide-co-glycolide)

PLGA is a copolymer of glycolic acid (GA) and lactic acid (LA) linked together by ester bonds. We can control the degradation rate of PLGA by adjusting various parameters such as LA / GA ratio, MW and structural polymer matrix. Based on these characteristics, we have developed PLGA nanoparticles (NPs) as carriers of RNAi delivery, which have the advantages of small particle size, high safety and sustained release.

• Chitosan

Chitosan is a copolymer of N-acetyl-D-glucosamine (GlcNAc) and D-glucosamine (GlcN). We can use positively charged chitosan to combine with siRNA with a negatively charged phosphate backbone to form a multi-stranded body, and its transmission efficiency is mainly affected by the weight ratio.

• Cyclodextrin

Cyclodextrin (CD) is a naturally occurring cyclic oligosaccharide. Due to its lack of immunogenicity, it has attracted much attention in the field of targeted therapy. CD-based synthetic delivery systems have proven some success in delivering nucleic acid payloads including pDNA, siRNA, and DNAzyme. The main strategy is to form an inclusion complex between the adamantane (AD) -containing molecule and the β-CD molecule. The modular linkage of the AD-PEG conjugate provides steric stability and the targeting ligand (AD-PEG-transferrin) confers cell-specific targeting.

Creative Biolabs has optimized the siRNA delivery process through the development of different polymer carrier systems to help customers achieve efficient and safe RNAi transfection. If you want to know more details, please contact us by email or send us an inquiry to find a complete solution.