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Single-Cell DNA Methylation Sequencing Service

Introduction Services Workflow Bioinformatics Advantages Samples Q&As Resource

Single-cell DNA methylation sequencing enables us to understand the heterogeneity of genome-wide 5-methylcytosine (5mC) patterns, providing a comprehensive and powerful platform for tumor diagnosis and precision therapy. As a leader in NGS-based cancer research, Creative Biolabs offers unrivaled single-cell whole-genome bisulfite sequencing services based on our advanced and powerful sequencing platforms.

Introduction of DNA Methylation

DNA methylation is an epigenetic modification that occurs by the addition of methyl (CH3) groups to the DNA molecule. It plays a key role in the regulation of gene expression without changing the gene sequence. In mammals, DNA methylation is associated with many key biological processes including genomic imprinting, X-chromosome inactivation, repression of transposable elements, aging, and carcinogenesis. Furthermore, DNA methylation is also associated with tumorigenesis. Increased evidence has reported aberrant DNA methylation is involved in the development and progression of human cancers. Tumor cells may display hypomethylation, leading to an increased oncogene activity which may be suppressed in normal cells. While tumor suppressor genes display hypermethylation which renders them inactive. Changes in DNA methylation in cancer can be considered as a promising target for the development of powerful diagnostic, prognostic, and predictive biomarkers.

Single-cell Whole Genome Bisulfite Sequencing

Although most cell types within an organism share an almost identical genomic DNA sequence, differences in DNA methylation patterns contribute to cell-specific gene expression and functional diversity. This epigenetic variation underlies cellular heterogeneity both across different cell types and within the same cell population. Single-cell whole-genome bisulfite sequencing combines bisulfite treatment and high-throughput sequencing technologies to detect genome-wide methylation at a single-cell level, making it possible to explore the epigenetic information of highly heterogeneous cells.

Single-cell bisulfite sequencing is an accurate and reproducible method for genome-wide methylation analysis. (Creative Biolabs Authorized)

Workflow

Fig.2 Single-cell WGBS seq workflow. (Creative Biolabs Original) Fig.1 Workflow diagram.

Bioinformatics

General Analysis	Advanced Analysis
QC for raw data QC for clean data Conversion efficiency evaluation Sequence alignment Differentially methylated sites or regions annotation Visual display for differentially methylated sites or regions	Analysis for hypomethylation and hypomethylation of all sites in the promoter region Analysis of the methylation status of CG sites in specific genes Trend analysis of specific gene methylation k-means clustering WGCNA analysis Customized analysis scheme

Our Advantages

Well-established single-cell whole-genome bisulfite sequencing technology
An experienced expert team providing one-stop services from preparation of cell suspension to high-quality data delivery
Personalized and customized data analysis services

Sample Requirement

Sample type: cell lines, primary cells, fresh tissue, cryopreserved cells
Species: Humans, mice, and other species need to be evaluated
Storage and transportation: Stored in liquid nitrogen, transported on dry ice

If you are interested in our services, please feel free to contact us for more details.

Q&As

Q: What are the advantages of single-cell DNA methylation sequencing over bulk sequencing?

A: Single-cell DNA methylation sequencing offers higher resolution compared to bulk sequencing, which averages methylation signals across many cells. This high resolution allows for the detection of rare cell populations and specific epigenetic changes that may drive cancer progression.

Q: Can single-cell DNA methylation sequencing be applied to all cancer types?

A: Yes, this technique can be applied to a wide range of cancer types. It is particularly useful for studying cancers with significant cellular heterogeneity, such as breast cancer, lung cancer, and hematological malignancies.

Q: How does single-cell DNA methylation sequencing integrate with other single-cell technologies?

A: Integrating single-cell DNA methylation sequencing with other single-cell technologies, such as RNA sequencing and chromatin accessibility assays, provides a comprehensive view of cellular states. This multi-omics approach helps in understanding the complex regulatory networks driving cancer.

Q: What challenges are associated with single-cell DNA methylation sequencing?

A: Challenges include technical complexities in cell isolation, low DNA input, and the need for advanced bioinformatics tools to analyze large datasets. Despite these challenges, ongoing technological advancements are improving the efficiency and accuracy of this method.

Q: How does single-cell DNA methylation sequencing contribute to personalized medicine?

A: By providing detailed methylation profiles of individual cancer cells, this technique helps in identifying unique epigenetic markers for each patient. These markers can guide personalized treatment strategies, improving therapeutic outcomes and minimizing side effects.