Transposon-mediated germline transgenesis has become a powerful tool to build genetically modified animal models as well as cell lines. At Creative Biolabs, we have successfully obtained transgenic rodent animals with large fragment insertion and high transgene efficiency by using different transposon systems, including PiggyBac, Tol2, and Sleeping Beauty. We are proud and willing to share our cutting-edge technology and rich experience with our clients to promote their brilliant studies.
Discovery of the PiggyBac Transposon
The PiggyBac transposon superfamily is relatively recently recognized as a widespread DNA transposon family with a broad host spectrum from yeast to mammals. This type of transposon was originally thought to be a rare transposon but now it has been deeply characterized by the discovery and isolation of PiggyBac-like elements from a number of species. Currently, the PiggyBac transposon is the most widely used transposon system for genetic manipulations in various fields.
Mechanisms of Transposition
The PiggyBac (PB) transposon system is composed of a transposable element, a gene of interest, and a transposase. Efficient transposon between vectors and chromosomes is mediated by the ‘cut and paste' mechanism. The target gene is inserted into the transposon vector between two inverted terminal repeat sequences (ITRs), which are transposon-specific sequences located on both ends of the vector. During transposition, the PB transposase recognizes ITRs and cut the target sequences, thereby moving the contents to the target sites and integrating them into TTAA chromosomal sites randomly dispersed in the genome. In general, all members of the PiggyBac superfamily use TTAA as their integration target sites.
Features of PB Transposon
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