The Tol2 transposon element was originally identified in the genome of the medaka fish and has now been extensively used to make transgenic zebrafish model for various studies. Afterward, this Tol2 transposon was discovered to be active in many other vertebrates, including zebrafish, chicken, mouse, human and so on. However, as an alternative way of DNA microinjection, Tol2 has not yet been widely used in mouse transgenesis. At Creative Biolabs, this technology has been successfully used to create transgenic mice with high efficiency and large fragment insertion.
Types of Tol2 Transposon
Generally, wild-type transposons exist as both ‘autonomous' and ‘non-autonomous' versions. The Tol2 element is an autonomously active transposon, containing a gene encoding a complete and functional transposase that is capable of identifying, excising, and reinserting the DNA element defined by its inverted terminal repeats (ITR) or other elements with the same ITRs. Non-autonomous elements, which are no longer able to produce their own transposase protein due to mutations or deletions of the coding region of the transposase, can also be catalyzed by this functional enzyme.
Features of Tol2 Transposon System
Applications of Tol2 Transposon System
Due to the high efficiency and abilities to carry larger foreign DNA fragments, Tol2 transposon system is discovered to be useful in not only the establishment of stable expression cell strain but also the generation of transgenic mice and rats. By injecting a foreign DNA cloned into a Tol2-transposon vector together with the transposase mRNA into the cytoplasm of fertilized eggs, we can obtain the Founder mice simply and easily. In addition to Tol2 transposon, other types of transposon-mediated transgenic services including PiggyBac and Sleeping Beauty transposons are also included in our list.
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