Owing to wide distribution in all body fluids and their critical functions in cell-cell communication, exosomes have always been research hotspots in recent years. Equipped with state-of-art tools and technologies, Creative Biolabs spares on effort to provide high-quality exosomal mRNA microarray services for rapid and comprehensive exosome mRNA profiling.

Gene Microarray

Gene microarray, commonly known as gene chip, is a biological detection technology developed in the 1980s-1990s. In a gene microarray, thousands of nucleic acid probes are fixed on a special material with an area of only a few square centimeters. Based on the principle of nucleic acid complementary pairing and DNA hybridization, the DNA, cDNA, and RNA to be tested in the sample can be attached to the nucleic acid probes on the chip and detected by fluorescence or current. A large number of gene sequence information can be qualitatively or quantitatively determined through one gene microarray test. Over these decades, multiple types of gene microarrays have been developed and extensively applied for the gene analysis of human diseases, being useful tools for genomics and genetics research.

Fig.1 An overview of DNA microarray technology. (Maia, 2018)

Exosomal mRNA Microarray

Exosomes are nanoscale membrane vesicles formed by the inward budding of cytoplasmic membranes, enveloping abundant contents such as proteins, nucleic acids, and other cellular metabolites. These exosomal cargoes can be transferred to adjacent or specific recipient cells through a series of endocytic pathways, thus affecting the activities and behaviors of recipient cells. Exosomal mRNA microarray is a high throughput and reliable method to profile mRNAs in exosomes. Generally, before performing the microarray, it should first isolate exosomes and their mRNAs from the target body fluids. Then, the extracted mRNAs are converted into cDNA using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), constructing the cDNA library. Add the cDNA library to a DNA microarray chip, the exosomal cDNA complementary sequence will be hybridized with the pre-labeled probe on the chip. The exosomal mRNA profiles can be visualized and analyzed by detecting the fluorescence intensity of microarray probes.

Fig.2 Exosomal mRNA microarray.

One-Stop Exosomal mRNA Microarray at Creative Biolabs

As a professional research organization, Creative Biolabs is committed to providing high-level and comprehensive exosome services to advance exosome-based therapy development. We are an experienced supplier in offering professional technical support and one-stop exosome services for research demands. In addition to exosomal mRNA microarray tests, an array of support products and services are also available, which mainly including but not limited to:

• Convenient Tools for Exosome Isolation
• Exosome Engineering
• Exosome Proteomics Services
• Exosome Lipidomics & Metabolomics Services

Please do not hesitate to contact us for technical support and tailored solutions if there is no option for the exosome service you're interested in.

Reference

1. Afzal, M.; et al. A fast and reliable pipeline for bacterial transcriptome analysis case study: serine-dependent gene regulation in Streptococcus pneumoniae. Journal of Visualized Experiments. 2015, 98: e52649.

• Exosomal Small RNA/miRNA Sequencing
• Exosomal lncRNA Sequencing
• Exosomal circRNA Sequencing
• Exosomal mRNA Sequencing
• Exosomal miRNA Microarray
• Exosomal LncRNA Microarray
• Exosomal ceRNA Microarray
• Exosomal Whole Transcriptome Sequencing