Potato-derived Exosome Research and Application

Potato-derived exosomes have been shown to exhibit similar biophysical characteristics to exosomes from other sources. Further research and application exploration of potato-derived exosomes is still ongoing. Creative Biolabs specializes in providing comprehensive research services related to potato-derived exosomes to support an improved understanding of potato-derived exosomes.

Isolation of Potato-derived Exosomes

Removal of sprouted or green potatoes from raw materials
Wash potatoes with distilled water and PBS and peel them properly to avoid soil and bacterial contamination
Obtain potato juice through a squeezer.
Collect the potato supernatant after low-speed centrifugation and discard large potato pieces.
Differential centrifugation cleared the potato extract and then filtered it.
Centrifuge potato juice at ultra-high speed.
Resuspend the potato exosome pellets with PBS.

Research on Potato-derived Exosomes

Research	Conclusion
Characterization of potato-derived exosomes.	Potato-derived exosomes are 50-250 nm in size, with elliptical bilayer morphology, and stable in solution with negative electronegativity.
Potato-derived exosomes are capable of entering keratin-forming cells.	After co-incubation of green fluorescently labeled potato-derived exosomes with keratinocytes, the presence of fluorescent signals representing potato exosomes in the cells was observed under confocal microscopy. Moreover, it was further revealed by flow cytometry that potato-derived exosomes were taken up by keratinocytes in a concentration-dependent manner.
Potato exosomes regulate inflammation in keratinocytes.	RT-PCR detected a more significant reduction in the expression levels of MMP and inflammatory cytokine genes in keratinocytes treated with potato-derived exosomes compared to those treated with exosomes from other sources, such as pear and citrus, suggesting the possibility of investigating the anti-photo-aging potential of potato exosomes.
Potato exosomes promote keratinocyte proliferation.	Cell proliferation and cytotoxicity assays revealed that potato exosome treatment brought negligible cytotoxicity to keratinocytes. In addition, the proliferation of keratinocytes increased with the concentration of potato exosomes.
Potato-derived exosomes prevent oxidative stress.	In a model of H₂O₂-induced oxidative stress in keratinocytes, cells pre-treated with potato-derived exosomes exhibited antioxidant properties. Colorimetric results revealed that potato exosomes exerted clear free radical activity in a concentration-dependent manner.
Potato exosomes mitigate cellular photodamage.	Potato-derived exosomes exert a dual protective effect of prevention and rescue of photodamaged keratinocytes by inhibiting the expression of inflammatory cytokines and unfavorable MPP for collagen synthesis, while preventing cellular peroxidation by up-regulating the level of glutathione S-transferase alpha 4. In addition, no other components of potato origin were detected to act in this way, demonstrating that it is indeed the potato-derived exosome that exerts the specific activity.

Mechanisms of potato-derived exosomes protecting cells from photodamage. (Lee, et al., 2023)

Fig. 1 Mechanisms of potato-derived exosomes protecting cells from photodamage.¹

The beneficial effects of potato-derived exosomes on cell protection and amelioration have been found in research studies, suggesting the potential application of potato-derived exosomes in skin care and wound repair. Creative Biolabs is equipped with proven exosome research platforms and specialized teams to assist clients with a variety of studies on potato-derived exosomes. Please contact us to discuss your expectations.