Nervous System Disease Model Construction Service for Exosome Functional Research

Overview Disease Model FAQs

Overview

Exosome research is an emerging and rapidly developing field that has received increasing attention. In existing studies, exosomes have played a significant role in targeting, immunogenicity, and carrying biologically active molecules, making them play an important role in the treatment of nervous system diseases (NSDs). These suggest that researchers can start from the direction of exosomes to find new therapeutic avenues. During the development of therapeutic exosomes, the selection of appropriate animal models is helpful for the development of effective therapeutic drugs. Creative Biolabs can provide a variety of stable and high-quality NSD animal models to help customers achieve their goals.

Fig.1 Research diagram of the mechanism of tanshinone IIA to improve cognitive function via synaptic plasticity in epileptic rats. (Jia, 2023)

Creative Biolabs Nervous System Disease Model Library for Exosome Functional Research

We can provide including but not limited to the following NSD animal models for exosome functional research.

NSD Animal Models	Inducer	Induction Mechanism	Applicable Animals	Model Features
Parkinson's disease (PD) animal models	6-hydroxydopamine (6-OHDA) induction	6-OHDA has strong neurotoxicity, can cause acute degeneration of sympathetic adrenergic nerve endings, and eventually cause irreversible damage to neurons.	Rat	This model is suitable for the study of the pathogenesis, treatment, and long-term observation of PD, especially for the study of pathophysiological changes in the middle and late stages of human PD.
	Rotenone induction	Rotenone is lipophilic, can pass through the blood-brain barrier, and has a strong and extensive inhibitory effect on the activity of mitochondrial respiratory chain complex I in brain tissue.	Rat	The model can better simulate PD-related characteristics in terms of pathology, biochemistry, pathogenic mechanism, and behavior.
	1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induction	MPTP is a natural insecticide, which is highly lipophilic and easily penetrates the blood-brain barrier. After entering the brain, MPTP can inhibit the activity of mitochondrial respiratory chain complex I, leading to the degeneration and death of dopaminergic neurons.	Mouse	The production cycle of this model is short, its neuron loss is obvious and the behavioral test is abnormal. It is currently one of the most commonly used PD animal models.
Alzheimer's disease (AD) animal models	APP/PS1 mice	APP/PS1 double transgenic mice express a fusion consisting of mutated presenilin (DeltaE9) and amyloid precursor protein (APPswe). β-amyloid deposits are produced in the brain of mice aged 6-7 months.	Mouse	This model is the most classic model for studying AD.
Alzheimer's disease (AD) animal models	3XTG mice	3XTG mice carry three AD-related genes, namely Psen1, APPswe, and tauP301L. At 12-15 months of age, this mouse had excess tau phosphorylated multimers in the hippocampus.	Mouse	This mouse exhibited plate and tangle pathology associated with synaptic dysfunction, which is similar to clinical AD pathology.
Epilepsy animal models	Combined induction of lithium chloride and pilocarpine	Pilocarpine activates acetylcholine receptors in the brain, causing persistent generalized tonic-clonic seizures. Lithium chloride can effectively increase the sensitivity of the body to pilocarpine, reduce the dosage of pilocarpine, and significantly reduce animal mortality caused by the toxic effect of pilocarpine.	Rat	The development process of this model is highly similar to that of human temporal lobe epilepsy, and it is an ideal model for studying temporal lobe epilepsy.

Creative Biolabs is a professional exosome research technology supplier, which can provide one-stop services including exosome extraction, exosome identification, exosome engineering, exosome labeling, and in vivo and in vitro verification of exosomes. With the support of a strong high-level team and the support of senior biotechnology experts, we can provide high-quality NSD animal models and supporting experimental services for global customers. Please contact us to put forward your needs to customize a rigorous experimental plan for you.

Reference

Jia, C.; Zhang, R.; et al. Investigation of the mechanism of tanshinone IIA to improve cognitive function via synaptic plasticity in epileptic rats. Pharmaceutical Biology. 2023. 61(1):100-110.

FAQs

What role do exosomes play in nervous system diseases, and how can they be harnessed for disease modeling?

Exosomes are implicated in the pathogenesis of various nervous system diseases, including neurodegenerative disorders, stroke, and brain tumors. Our service utilizes exosomes to construct disease models that replicate key pathological features, providing valuable platforms for studying disease mechanisms and testing therapeutic interventions.

How are exosomes sourced for nervous system disease model construction, and what advantages do they offer in this context?

Exosomes can be isolated from cerebrospinal fluid, blood, or neuronal cells, serving as reservoirs of disease-specific biomarkers and mediators. Utilizing exosomes for disease modeling offers advantages such as physiological relevance, non-invasiveness of sample collection, and the ability to capture disease-specific molecular signatures for accurate modeling.

Which nervous system diseases can be modeled using exosomes, and how faithfully do these models mimic human pathophysiology?

Our service enables the modeling of various nervous system diseases, including Alzheimer's disease, Parkinson's disease, stroke, and glioblastoma. These models closely mimic human pathophysiology by incorporating disease-associated exosomes containing biomolecules indicative of disease status, facilitating the study of disease progression and therapeutic responses.

What methodologies are employed in constructing nervous system disease models using exosomes, and what experimental approaches are involved?

Nervous system disease models are constructed by administering disease-associated exosomes to relevant in vitro or in vivo model systems, such as neuronal cultures or animal models. Experimental approaches include exosome uptake assays, functional assays, histological analysis, and molecular profiling techniques to assess disease-relevant phenotypic changes and therapeutic responses accurately.

Can these exosome-based disease models be customized to address specific research questions or therapeutic targets?

Yes, our service offers customizable disease models tailored to address specific research questions, therapeutic targets, or experimental requirements. Researchers can choose disease-relevant exosome sources, model systems, disease endpoints, and outcome measures, allowing for flexible study designs and the exploration of diverse research avenues.

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