Urinary System Disease Model Construction Service for Exosome Functional Research

Overview Disease Model FAQs

Overview

At present, the diagnosis and treatment of most urinary system diseases (USDs) lack specificity and sensitivity, which can easily cause delays in the disease and other unnecessary damage to the body. Therefore, it is important to find better ways to diagnose and treat USDs. In recent years, with the development of targeted therapy technology, exosomes have gradually become an important target for disease diagnosis and treatment due to their good targeting and tolerance. A variety of cells can release exosomes, which are membranous vesicles with the function of carrying substances and transmitting signals, and are the communication media between almost all cells. A large number of studies have shown that natural exosomes or engineered exosomes have a good therapeutic effect on USDs. These findings are inseparable from a suitable USD animal model. Creative Biolabs has been paying attention to the research progress of USD-related exosome therapy and diagnosis and has been updating its technology and knowledge reserves. We can provide customers with the most suitable USD animal model and one-stop services including exosome extraction, exosome identification, exosome engineering, exosome labeling, and in vivo and in vitro verification of exosomes.

Fig.1 Schema of generation of the Unilateral ureteral obstruction (UUO) model. (Belghasem, 2019)

Creative Biolabs Urinary System Disease Model Library for Exosome Functional Research

We can provide including but not limited to the following USD animal models for exosome functional research.

USD Animal Models	Method	Modeling Mechanism	Applicable Animals	Model Features
Renal fibrosis animal models	Unilateral ureteral obstruction	After the unilateral ureter is ligated, urine retention compresses the renal tubules, leading to progressive necrosis of renal tubular epithelial cells and infiltration of inflammatory cells. Subsequently, the necrotic tubular tissue is gradually replaced by fibrous scarring, culminating in progressive renal fibrosis.	Rat	This model is a relatively mature model of renal fibrosis, and fibroblast proliferation and extracellular matrix can be produced 2 weeks after ligation. The pathological features of this model are similar to those observed in patients with renal fibrosis.
Renal fibrosis animal models	5/6 nephrectomy	After 5/6 of the kidney was removed, the mice exhibited mesangial matrix hyperplasia, glomerulosclerosis, focal atrophy of the renal tubules, and interstitial fibrosis. These manifestations are a pathological process consistent with human renal fibrosis.	Rat	In this model, the residual renal tissue is overloaded while the residual renal tissue is relatively normal, and the influence of various pathogenic factors of primary kidney diseases on the residual nephron is excluded, and the influencing factors are simplified.
Acute kidney injury animal models	Combined induction of renal ischemia-reperfusion and contralateral nephrectomy	This method blocks the blood supply to the kidney, damages the kidney function, causes cellular ischemia and hypoxia, and finally causes damage to the renal tubular epithelial cells, inducing acute kidney injury.	Rat	The pathological damage in this model is significant and progressive.
Acute kidney injury animal models	Adriamycin induction	When doxorubicin acts on the kidney, it can lead to abnormal cytoskeleton structure, damage to the integrity of the glomerular filter membrane, and up-regulation of various inflammatory factors.	Mouse, rat	This model is currently recognized as a model that can better simulate human acute kidney injury.
animal models	Adenine induction	Adenine generates 2,8-dihydroxyadenine through the action of xanthine oxidase, and the latter is deposited in the glomerulus and renal interstitium, forming foreign body granulomatous inflammation, and causing cystic dilatation of renal tubules by blocking the lumen of the renal tubules. As the disease progresses, a large number of nephrons are lost leading to renal failure.	Rat	According to the size of the adenine dose and the length of feeding time, mild, moderate, and severe renal failure models can be made. The model has a stable disease course, is suitable for long-term administration, and has a high success rate.

Creative Biolabs has more efficient experimental facilities and richer project experience and can provide more USD animal models for customers to choose from. Please feel free to contact us with your needs. Our professional team will design a more reasonable experimental plan for you to accelerate the progress of your project.

Reference

Belghasem, ME.; A'amar, O.; et al. Towards minimally-invasive, quantitative assessment of chronic kidney disease using optical spectroscopy. Scientific Reports. 2019. 9(1):7168.

FAQs

What is the relevance of exosomes in urinary system diseases, and how can they be utilized for disease modeling?

Exosomes play crucial roles in the pathogenesis of urinary system diseases, including kidney disorders, urinary tract infections, and bladder cancer. Our service leverages exosomes to construct disease models that mimic key pathological features, providing valuable platforms for studying disease mechanisms and evaluating therapeutic strategies.

How are exosomes sourced for urinary system disease model construction, and what advantages do they offer in this context?

Exosomes can be isolated from urine, blood, or kidney tissue, serving as rich sources of disease-specific biomarkers and mediators. Utilizing exosomes for disease modeling offers advantages such as non-invasiveness of sample collection, physiological relevance, and the ability to capture disease-specific molecular signatures, facilitating accurate disease modeling and therapeutic development.

Which urinary system diseases can be modeled using exosomes, and how accurately do these models recapitulate human pathophysiology?

Our service enables the modeling of various urinary system diseases, including chronic kidney disease (CKD), nephrotic syndrome, urinary tract infections (UTIs), and renal cell carcinoma (RCC). These models closely mimic human pathophysiology by incorporating disease-associated exosomes containing biomolecules indicative of disease status, allowing for the study of disease progression and therapeutic responses.

What methodologies are involved in constructing urinary system disease models using exosomes, and what experimental approaches are employed?

Urinary system disease models are constructed by administering disease-associated exosomes to relevant in vitro or in vivo model systems, such as cell cultures or animal models. Experimental approaches include exosome uptake assays, functional assays, histological analysis, and molecular profiling techniques to assess disease-relevant phenotypic changes and therapeutic responses accurately.

Can these exosome-based disease models be tailored to address specific research questions or therapeutic targets?

Yes, our service offers customizable disease models tailored to address specific research questions, therapeutic targets, or experimental requirements. Researchers can choose disease-relevant exosome sources, model systems, disease endpoints, and outcome measures, allowing for flexible study designs and the exploration of diverse research avenues.

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