Exosome-based delivery system has ideal advantages, making it a superior choice, such as specificity, stability, long circulating half-life and biocompatibility with minimal toxicity to target tissue. At present, Creative Biolabs has successfully realized the loading of cargos into exosomes by using the pre-loading strategy.
Loading cargo into exosomes requires bypassing the barrier of the outer membrane. Synthetic carriers such as liposomes can be loaded with cargos during the synthesis process. Similarly, exosomes can be pre-loaded in situ by adding exogenous drugs to parental cells. Co-expressed proteins in parental cells can also be guided into exosomes through designed protein-protein interactions. In the pre-loading technique, the exosomes are loaded with cargos indirectly, that is to say, the parental cells are incubated with cargos at room temperature, and the selected proteins or drugs can be loaded in advance. When the cells release exosomes, these vesicles will be loaded with cargos. This strategy is particularly conducive to the loading and delivery of oligonucleotides.
Fig.1 Exosome generation, secretion and cargo transfer from the donor cells to the recipient cells. (Luan, 2017)
Due to the availability of various cellular engineering methods, most of the modification of exosomes has been performed on parental cells, which are then cultured to secrete the modified exosomes. Possible pre-loading strategies include:
Protein sequences and different types of RNA are commonly used for cell transfection, ultimately altering the phenotype of the released exosomes. Genetic engineering strategies may require the use of lipid methods (i.e., via Lipofectamine) to load cargo or produce the desired gene expression. By changing the synthesis of exosomes, people can control the therapeutic cargo they carry.
Fig.2 Exosomes can be modified by manipulating progenitor cells. (Xu, 2020)
Exosomes can transfer encapsulated proteins and genetic information to recipient cells and act as information transmitters between cells. Creative Biolabs has successfully established the pre-loading strategy to efficiently load exogenous cargos into exosomes. If you are interested in our services, please contact us to discuss your specific project requirements.
References
Exosome cargo pre-loading involves loading therapeutic cargo into exosomes during their biogenesis within donor cells, prior to their secretion. This approach offers advantages in cargo stability and loading efficiency compared to post-secretion loading methods, ensuring uniform cargo distribution within exosomes.
Exosome cargo pre-loading ensures efficient encapsulation of therapeutic cargo within exosomes, enhancing cargo stability and protecting it from degradation. This promotes sustained release and targeted delivery of therapeutics to recipient cells, ultimately improving therapeutic efficacy and reducing off-target effects.
Yes, our exosome cargo pre-loading service offers customization options to meet diverse research needs. Researchers can select specific cargo molecules, loading methods, and donor cell types to tailor exosome formulations for their desired therapeutic or diagnostic applications.
Exosome cargo pre-loading offers several advantages over post-secretion loading techniques, including improved cargo stability, uniform cargo distribution within exosomes, and enhanced loading efficiency. Moreover, pre-loaded exosomes exhibit enhanced bioavailability and therapeutic efficacy, making them ideal candidates for drug delivery applications.
Researchers can evaluate the efficacy of exosome cargo pre-loading by assessing cargo loading efficiency, release kinetics, and therapeutic effects in relevant cell culture models or animal studies. Additionally, monitoring changes in cellular pathways or biomarkers associated with therapeutic responses provides insights into exosome-mediated effects in target cells.