Tandem diabody is a type of bispecific antibody fragments. It is a tetravalent bispecific molecule, 2+2 antigen-binding valency, consisting solely of Fv domains. Tandem diabody is expressed as a monomeric subunit (single chain Diabody, scDb) with four variable domains from two parental antibodies. In the subunit, the linkers are too short to form intra-chain pairing, so the 2 polypeptides dimerize in a head-to-tail fashion. Some tandem diabodies have been studied in tumor immunotherapy.
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