Human LIGHT Recombinant Cell Line, CHO-K1 (CAT#: IOC-LX116)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry. Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

This cell line is useful for LIGHT binding assays, flow cytometry, or for screening for LIGHT antibodies. Also useful for immune checkpoint co-stimulatory assay.

Human

CHO-K1

LIGHT

LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTβR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-κB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface.