Description
This kit has been developed to isolate highly purified human CD31+ cells from whole blood, buffy coat, or peripheral blood mononuclear cells (PBMCs). The CD31 Fab is a recombinantly expressed low affinity Fab fragment. The CD31 Fab-Strep binds to the matrix of Strep-Agarose columns. Therefore CD31+ target cells can adhere to the matrix. Finally, the addition of biotin causes the elution of the target cells from the matrix, and Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
Highly pure cells;
Label-free cells.
Applications
Cell Isolation
Application Notes
Desired cells are labeled with low affinity CD31 Fab.
Comment
This kit is available for processing 4 x 10^7 cells.
Method Type
Positive selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Whole blood, buffy coat or PBMCs
Datasheet