Description
This kit has been developed to isolate highly purified CD4+ T cells from mouse spleen by negtive selection. Isolated cells are immediately available for downstream applications such as flow cytometry, phenotypic characterization, culture, or cell-based experiments.
Features
Fast and easy workflow takes only 30 minutes;
No magnet, no column, no harsh chemicals.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and depleted.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr-1, TCR‑γδ and TER-119.
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Datasheet