LS411N In Vitro Reporter Gene (β-glucuronidase) Assay (CAT#: ITS-1122-YF520)

LS411N

Human

LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes' B, poorly differentiated cecal carcinoma.
The cells obtained after passage in nude mice are referred to as LS411N cells.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Reporter Gene (β-glucuronidase) Assay

LS411N-cell based In Vitro Reporter Gene (β-glucuronidase) Assay

Reporter gene assays have become a valuable technique for studying gene expression by both cis- and trans-acting regulatory factors. The action of a reporter gene is evaluated in a reporter gene assay and a reporter gene construct normally comprises of a target regulatory DNA sequence (usually a major in a cancer pathway) to be analyzed, sequence for the reported gene and a sequence for functional mRNA in an expression vector. The expression vector can be transfected into the desired cell type. After transfection of the vector, the effects on a drug on the expression of target gene can be evaluated. The ideal reported gene activity should detect with high sensitivity over any endogenous gene expression. Reporter gene systems permit the use of cancer pathway-specific or developmentally regulated regulatory DNA sequences as markers to analyze gene expression in specific pathways associated with molecular mechanisms of cancer.

β-glucuronidase

High-throughput Screening, High Content Screening and Reporter Gene Assays