PathFinder™ CSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway) (CAT#: IOK-LX091)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. The CSF1R SRE Reporter Kit is designed for monitoring the activity of the CSF1R signaling pathway in cultured cells. The kit contains a transfection-ready vector for CSF1R and SRE luciferase reporter vector. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

Available to study immunology signaling pathway, monitor and screen small molecular.

• Monitor CSF1R/MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway.
• Study effects of RNAi or gene overexpression.

MAPK/ERK Signaling Pathway

Cell Signaling Pathway, Immunology, Onconology