Description
The PathFinder™ Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.
Applications
Biosimilar & Biobetter Bioassay
INN Drug Name
Epoetin Alfa
Molecular Mechanism of Action
EPOR agonist
Qualified With
Recombinant hEpo
Assay Measures
EpoR/EpoR Dimerization
Datasheet