Short Decsription
FGL1: LAG3 TR-FRET assay is designed to measure the inhibitory effect of a homogeneous 96 or 384 reaction format on the binding of LAG3 to FGL1. This TR-FRET-based assay does not require time-consuming washing steps, so it is particularly suitable for high-throughput screening applications. The measurement process is simple and straightforward. Incubate samples containing biotinylated LAG3, His-tagged FGL1 protein and inhibitor for one hour. Then, the anti-His Tb donor and dye-labeled acceptor were added, and the fluorescence intensity was measured using a fluorescence reader.
Description
The FGL1:LAG3 TR-FRET Assay is designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated LAG3, His-tagged FGL1 protein, and an inhibitor are incubated for one hour. Then, anti-His Tb donor and dye-labeled acceptor are added and fluorescence intensity is measured using a fluorescence reader.
Applications
This kit is useful for screening for inhibitors of LAG3 binding to FGL1.
Target
HFREP1, HP-041, LFIRE-1, LFIRE1, fibrinogen like 1
Datasheet