PathFinder™ LSD1 Inhibitor Screening Assay Kit (CAT#: IOK-LX078)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. LSD1 Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening LSD1-specific inhibitors. The assay is based on the multistep enzymatic reaction in which LSD1 first produces H2O2 during the demethylation of lysine 4 on a peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3. In the presence of horseradish peroxidase, H2O2 reacts with ADHP to produce the highly fluorescent compound resorufin that can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

Available to monitor and screen small molecular inhibitors for drug discovery and HTS applications, as well as enzyme kinetics study.

This kit is useful for screening small molecular inhibitors for drug discovery and HTS applications.

Lysine-Specific Demethylase 1

Immunology, Onconology