PathFinder™ Mouse Notch1 Pathway Reporter Kit (Notch Signaling Pathway) (CAT#: IOK-LX096)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for mouse NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular regions intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.

Available to study immunology signaling pathway, monitor and screen small molecular.

• Screen for activators or inhibitors of the Notch signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the Notch pathway.

Notch Signaling Pathway

Cell Signaling Pathway, Immunology, Onconology