PathFinder™ PD-1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit (CAT#: IOK-02P084)

After binding to the PD-L1 ligand, the cellular signal sent through the PD-1 receptor weakens the immune response and is used by tumors and viruses. PD-1 [Biotinylation]: The PD-L1 inhibitor screening and analysis kit is designed to screen and analyze inhibitors of this signal transduction. The kit has a convenient 384-well format, biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP and 400 binding reaction assay buffer. The key to this kit is the high sensitivity of the detection of biotin-labeled PD-1 through streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. First, apply PD-L1 on a 384-well plate. Next, PD-1 was incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP, and then HRP substrate is added to generate chemiluminescence, which can then be measured using a chemiluminescence reader.

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. This kit comes in a convenient 384-well format with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 400 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 384-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

This kit is most useful for screening for inhibitors of PD-L1.