Short Decsription
PD-1 / PD-L1 inhibitor screening cell-based assay is a bioluminescent cell-based assay that can be used to screen and analyze inhibitors of PD-1:PD-L1 interaction. The assay includes two main components: growth arrested PD-1 effector cells (PD-1/NFAT reporter-Jurkat cells): reporter Jurkat T cells express the firefly luciferase gene under the control of the NFAT response element, and also constitute Sexually express human PD-1. These cryopreserved cells are provided in the form of thawing and use, without the need for cell reproduction. These cells have been modified so that they are not expanded and are intended to be used in a single experiment. Expression vector for TCR activator and human PD-L1: A transfection-ready vector is used to transfect cells to produce target cells that overexpress PD-L1 and engineered cell surface T cell receptor (TCR) activator.
Description
The PD-1/PD-L1 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L1 interaction. The assay consists of two main components:; Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment.; Expression vectors for TCR activator, and Human PD-L1: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L1 and an engineered cell surface T cell receptor (TCR) activator.
Applications
Screen for activators or inhibitors of PD-1 signaling in a cellular context Characterize the biological activity of PD-1 and its interactions with ligands
Target
Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1
Datasheet