PathFinder™ RANK:RANKL TR-FRET Assay, 96 reactions (CAT#: IOK-02P093)

RANK: The RANKL TR-FRET assay is designed to measure the inhibitory effect of RANK and RANKL binding. This FRET-based assay does not require time-consuming washing steps, so it is particularly suitable for high-throughput screening applications. The measurement process is simple and straightforward. Incubate samples containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and inhibitor for one hour. Then, the fluorescence intensity is measured using a fluorescence reader.

The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for one hour. Then, the fluorescence intensity is measured using a fluorescence reader.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

RANK, RANKL, TNFRSF11A, TNF Receptor Superfamily Member 11a, TNF Superfamily Member 11, TNFSF11, TNF Receptor Superfamily Member 11b, TNFRSF11B, HTRF