PathFinder™ SIRT1 FRET-Based Screening Assay Kit (CAT#: IOK-LX084)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. SIRT1 FRET-based Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence can be easily analyzed using an excitation wavelength of 335-345 nm and emission wavelength of 440-465 nm.

Available to monitor and screen small molecular inhibitors for drug discovery and HTS applications, as well as enzyme kinetics study.

This kit is useful for screening small molecular inhibitors for drug discovery and HTS applications.

SIRT1

Immunology, Onconology