Description
This kit has been developed to isolate highly purified plasma from human peripheral blood mononuclear cells (PBMCs) or bone marrow. Isolated cells are immediately available for downstream applications such as flow cytometry, signal transduction pathways analysis, or functional assays.
Applications
Cell Isolation
Application Notes
The first magnetic separation (depletion); The second magnetic separation (positive selection).
Comment
This kit is available for processing 2 x 10^9 cells.
Molecular Mechanism of Action
Non-plasma cells are removed by immunomagnetic depletion; Plasma cell are labeled with CD38 beads and isolated by positive selection.
Method Type
Depletion; positive selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
PBMCs or bone marrow