Amino acids play critical roles in the process of metabolic reprogramming to support cancer cell survival and growth, as well as tumor progression. For example, cancer cells enhance glutamine uptake through special transporters and use it to support their growth and survival. Cancer cells increase arginine metabolism and utilize arginine to produce proteins. Cancer cells convert tryptophan into kynurenine which can activate signaling pathways in cancer cells to promote cell proliferation and survival. It can also regulate the immune response in the tumor microenvironment, allowing cancer cells to evade immune surveillance. Additionally, kynurenine can regulate the production of reactive oxygen species (ROS).
Fig.1 Amino acid metabolic reprogramming in cancer.1
At Creative Biolabs, we provide tumor amino acid metabolism analysis services which are a comprehensive and targeted analysis of the tumor amino acid metabolic process. We are able to analyze and quantify a wide range of amino acid metabolites and provide detailed information on the changes in amino acid metabolism in tumors. The service includes a comprehensive analysis of tumor cells, tumor tissue samples, blood samples, and other relevant biological samples.
At Creative Biolabs, we offer comprehensive amino acid metabolome profiling services. Utilizing cutting-edge mass spectrometry-based techniques, we have the ability to analyze and quantify a wide range of amino acid metabolites in biological samples. This profiling service provides a comprehensive and holistic understanding of the metabolic state and dynamics of amino acids within the sample. We provide targeted amino acid metabolomics for a focused and precise analysis of specific amino acids or a subset of amino acids.
Creative Biolabs also offers comprehensive amino acid metabolic enzyme profiling services. This service utilizes western blotting, IHC analysis, proteomics, and bioinformatics techniques to identify and characterize the enzymes involved in amino acid metabolism. By analyzing the expression levels and activities of these enzymes, we gain insights into the regulation of amino acid metabolism in tumor cells.
Our multi-omics services for amino acid pathway analysis combine our targeted amino acid metabolomics data with other omics technologies, such as genomics, transcriptomics, and proteomics, to provide a more comprehensive understanding of correlations or interactions between genes, genomic variations, and metabolites and thereby providing a more holistic view of a biological system.
Method Used: Metabolomics
Journal: Scientific reports
Research Overview: This article utilized a metabolomic approach to reveal specific metabolic profiles of PANC-1 cells when the glycolytic pathway was inhibited. The results showed that the PANC-1 cells re-program their metabolic pathways and mitochondria dependent cell metabolism and amino acid metabolism changed dynamically.
Fig.2 Analysis of amino acid metabolism in PANC-1 cells.2
References
For Research Use Only | Not For Clinical Use
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