Components
Coating antibodies (to determine total number IgG secreting B cells); Biotinylated detection antibodies; B cell stimulus; Streptavidin-HRP conjugate; AEC coloring system; Blocking stock solution; Dilution buffer R; Tween-20;
Assay Procedure
See manual for details.
Assay Measures
B-cell ELISpot assay is used to determine the number of single antibody-producing memory B cells in a single-cell suspension. A major advantage of this method is its ability to activate antigen-specific memory B cells in vitro and then measure them.
Storage
Depends on the product components.
Storage Comment
Store protected from light.
Restrictions
For research use only. Not for use in diagnostic procedures.