PathFinder™ PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit (CAT#: IOK-02P087)

After binding to PD-L1 ligand, the cell signal transduction through PD-1 receptor; weakens the immune response, and is used by tumors and viruses. PD-; 1 [Biotinylation]: PD-L1 inhibitor colorimetric screening assay kit for screening; inhibitor of PD-1 receptor binding to PD-L1 ligand. The kit has a convenient 96-well; format, biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP and assay buffer; 100 binding reactions. The key to this kit is that biotin-labeled PD-1 has high affinity. Streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. The first one is; PD-L1 is coated on a 96-well plate. Next, PD-1 was incubated with PD-L1 on the plate. Finally, treat the plate with streptavidin-HRP, and then add a colorimetric HRP substrate to it; produce color, which can then be measured using a UV/Vis spectrophotometer microplate; reader.

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand; attenuates immune responses and is exploited by both tumors and viruses. The PD-; 1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screening; inhibitors of PD-1 receptor binding to PD-L1 ligand. This kit comes in a convenient 96-well; format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for; 100 binding reactions. The key to this kit is the high affinity of biotin-labeled PD-1 for; streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First,; PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the; plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to; produce color, which can then be measured using a UV/Vis spectrophotometer microplate; reader.

This kit is most useful for screening for inhibitors of PD-L1.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1)