Creative Biolabs has developed sensitive immunogenicity assessment technology (SIAT) to help clients address potential drug immunogenicity issues. This technology is effective for a wide range of applications.
Learn More →IDO1 Assay Portfolio Service
Overview What We Can Offer Why Choose Us FAQs Customer Review Related Services Contact Us
Overview
Indoleamine-2,3-dioxygenase 1 enzyme (IDO1) is recognized as one of the most potent therapeutic opportunities to inhibit tumor growth. Multiple studies have shown that IDO1 is highly expressed in several types of human cancer. With advanced and high-end technologies, rich experienced scientists, Creative Biolabs is an excellent service provider in the field of tumor marker assay. After long years ahead to fully comprehend tumor markers, we launch our IDO1 assay portfolio service which can be useful for targeted cancer therapy and diagnosis.
Introduction of IDO1
IDO1, a 45 kDa cytosolic haem-containing enzyme, is located on chromosome 8 comprising 10 exonic regions and the promoter region contains several transcription factor-binding sites that have been identified by ChIP-sequencing. IDO1 is distributed in various organs, such as the lung, spleen, liver, kidney and brain, and expressed in many cells, including astrocytes, macrophages and dendritic cells. In addition, Tumor cells can also express widely and variably IDO1, such as the tumor microenvironment's innate immune cells and tumor-draining lymph nodes.
Fig.1 The structure of the IDO1 gene.1, 3
IDO1 Regulatory and Effector Signaling Pathways
The expression and function of IDO1 were regulated by multiple upstream pathways, including the JAK-STAT, RAS-PKC, NF-κB, and Kit signaling pathways. The downstream of IDO1 contains three effector pathways that transduce the effects of IDO1 activity, including the activation of GCN2, the inhibition of mTOR and the activation of AhR pathway. The above pathways mediate immunosuppression and neovascularization in the tumor microenvironment.
Fig.2 Regulation, function, and targeting of IDO1 in cancer.2, 3
A Multidimensional Approach to IDO1 Assessment
Our IDO1 assay portfolio service provides an integrated, one-stop solution for every stage of your drug development pipeline. We recognize that no single assay can provide a complete picture, and thus, our services encompass both biochemical and cell-based methods to deliver comprehensive and preclinically relevant data.
Partner with Creative Biolabs to unlock the full potential of your IDO1 inhibitor program and make a meaningful impact in the fight against cancer.
Why Choose Us?
Creative Biolabs leverages decades of experience and deep scientific expertise to deliver exceptional quality in every project. Our IDO1 assay portfolio service provides a seamless, integrated solution that generates reliable, reproducible data—helping you save both time and resources. With an in-house team of Ph.D.-level scientists who have specialized knowledge of the IDO1 signaling pathway and its role in immunosuppression, we understand the intricacies of inhibitor development. Combined with our advanced technology platforms, this expertise ensures precise and rigorously executed research.
Ready to accelerate your project's success? Contact us today for a detailed proposal on a tailored partnership.
FAQs
Q1: What's the main advantage of your IDO1 cell-based assay compared to a biochemical one?
A1: Biochemical assays are great for initial screening and measuring direct enzyme inhibition. But our cell-based assays give you a more realistic, physiological view—they take into account things like how well a compound gets into cells and what kind of downstream effects it has.
Q2: Can you work with small projects, or do you only handle large compound libraries
A2: Absolutely—we work with projects of any size. Our services are flexible, so we can design a plan that fits exactly what you need, whether you're testing one compound or a whole library.
Q3: What are some common challenges in IDO1 inhibitor development, and how does your service help overcome them
A3: One big hurdle is making sure a compound that works well in a test tube remains effective in living systems. Our combined use of biochemical and cell-based assays gives you a fuller picture of a compound's behavior, helping you focus on those with the best potential to succeed in vivo.
Customer Review
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Physiologically Relevant Data
The IDO1 cell-based assay provided a more realistic model than we could achieve in our own lab. The results gave us a clear indication of our compound's functional activity, which was a major advantage in our preclinical studies. - Sarah B***s -
Strategic Project Guidance
We were struggling to compare our lead compound to a known inhibitor. Creative Biolabs' team not only ran the comparison but also provided valuable insights into the differences in potency and mechanism of action. - Mike H***d
Related Services
To further support your research, we recommend the following complementary services:
Immunogenicity Prediction
Large-Molecule Drug Bioanalysis Service
Creative Biolabs provides cutting-edge large-molecule drug bioanalysis services to meet the diverse needs of therapeutic biological product development. Our expertise covers pharmacokinetics, toxicokinetics, and biomarkers, ensuring high-quality services for their clients.
Learn More →Contact Us
Creative Biolabs is your trusted partner in advancing immuno-oncology research. Our IDO1 assay portfolio service is designed to provide you with the precision, expertise, and high-quality data you need to succeed. Contact us today to learn how we can help you accelerate your drug discovery journey.
References
- Hornyák, Lilla, et al. "The role of indoleamine-2, 3-dioxygenase in cancer development, diagnostics, and therapy." Frontiers in immunology 9 (2018): 151. https://doi.org/10.3389/fimmu.2018.00151
- Liu, Ming, et al. "Targeting the IDO1 pathway in cancer: from bench to bedside." Journal of hematology & oncology 11.1 (2018): 100. https://doi.org/10.1186/s13045-018-0644-y
- Distributed under Open Access license CC BY 4.0, without modification.
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