Creative Biolabs specializes in providing innovative solutions for cancer research and therapy development. We provide cutting-edge intratumoral Treg Assay Service that enables comprehensive analysis and characterization of intratumoral T regulatory cells (Tregs). By utilizing advanced techniques and state-of-the-art technologies, our service provides valuable insights into the role of Tregs in the tumor microenvironment, aiding in the development of novel immunotherapeutic strategies.
FoxP3, a master regulator of Treg function, serves as a reliable marker for the identification and quantification of Tregs. The assay employs state-of-the-art techniques, including flow cytometry, immunohistochemistry, and molecular biology assays, to accurately assess the presence and functional status of intratumoral Tregs. By employing a multi-parametric approach, Creative Biolabs can provide detailed information regarding the frequency, phenotype, and functional characteristics of intratumoral Tregs, facilitating a comprehensive understanding of their role in tumor progression and immune evasion.
Fig.1 Analysis of Tregs in CRC patients. (Betts, et al., 2012)
Our tumor-infiltrating Treg phenotyping service follows a well-defined workflow designed to ensure accurate and reliable results. The workflow can be divided into the following key steps:
Sample Preparation
We start by collecting tumor samples, either through surgical resection or biopsy, ensuring proper preservation and handling to maintain sample integrity. Our experienced team then processes the samples to obtain single-cell suspensions, optimizing cell viability for subsequent analysis.
Treg Cell Enrichment
To isolate tumor-infiltrating Treg cells, we employ state-of-the-art cell sorting technologies. By utilizing specific markers, such as FOXP3, CD25, and CD127, we selectively enrich Treg cells from the heterogeneous tumor-infiltrating lymphocyte population.
Phenotypic Profiling
Once the Treg cells are isolated, we employ a range of cutting-edge techniques for their phenotypic characterization. Flow cytometry is utilized to analyze surface markers, such as CD4, CD8, and various immune checkpoint molecules.
Functional Assays
We perform functional assays to evaluate the immunosuppressive capabilities of tumor-infiltrating Treg cells. These assays include suppression assays, cytokine profiling, and proliferation assays, which provide insights into the regulatory mechanisms employed by Treg cells to inhibit the effector functions of other immune cells.
Creative Biolabs' Tumor-Infiltrating Treg Depleting Service offers a customizable approach to selectively eliminate intratumoral Tregs, thus unleashing the full potential of the immune system against tumors. This service encompasses various methods, such as antibody-mediated depletion, chimeric antigen receptor (CAR) T cell therapy, and small molecule-based approaches, each tailored to the specific needs of the client's research or therapeutic objectives. The expertise and experience of Creative Biolabs in developing innovative Treg-targeting strategies ensure high specificity and minimal off-target effects, paving the way for enhanced anti-tumor responses.
Cutting-edge Technologies
Customizability
Expertise and Experience
Creative Biolabs's Intratumoral Treg Assay Service offers a comprehensive and customizable solution for studying the role of Tregs in tumor biology and immunotherapy. Please feel free to contact us for more about our Treg related services.
For Research Use Only | Not For Clinical Use
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