LPS Analysis Service

Background What is LPS? LPS and Innate Immunity
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Lipopolysaccharides (LPS) are involved in the growth or survival of bacteria, the host-parasite interaction, the immunity response, and the toxic pathophysical reaction. As a result, LPS is important to know and describe for applications from vaccine design to biologic safety. Creative Biolabs is dedicated to meeting these demands and has developed a comprehensive and tailor-made LPS Analysis Service. This service combines advanced techniques like SDS-PAGE, Mass Spectrometry, and ELISA with unique solutions like Recombinant Factor C Assays and LAL Assays for unprecedented accuracy of LPS endotoxin identification and endotoxin profiling for drug discovery.

Background

What is LPS?

LPS (or endotoxins) are essential to Gram-negative bacteria's structure and activity. Because they are strong stimulators of the immune system, they provide invaluable information about bacterial evolution and host immunity. However, LPS has a dual nature— beneficial at low levels but harmful in excess, thus exact characterization for them is important for both research and drug development. LPS has three main regions:

The Structural Properties of LPS.1,3Fig.1 The Structure of LPS.1,3
  • Antigen: As the most variable part of the bacteria, the O-antigen can change its composition to enable bacteria to deter immune recognition and evade host defenses.
  • Core Polysaccharide: this area is between lipid A and the O-antigen, protecting the structure and helping the bacterium survive.
  • Lipid A: The main driver of LPS' immunostimulatory action is this hydrophobic anchor. Its architecture directly affects the immune system, and this is why toxicology and therapeutic studies focus on it.

LPS and Innate Immunity

LPS is an effective alarming signal for Gram-negative bacteria infection, playing a crucial role in triggering the body's natural immune system. When LPS attaches to the Toll-like receptor 4 (TLR4) complex that contains the MD-2 co-receptor, a cascade of intracellular signaling pathways kicks in. These pathways (especially NF-B and MAPK) trigger the release of pro-inflammatory cytokines like TNF-α, IL-6, and IL-1β. This accelerated immune response is essential to activate defenses against bacteria, killing the bacteria effectively. But the potency of LPS also comes at a cost. Too much of it overstimulates immune cells, which can induce a dangerous inflammatory response, such as septic shock. The role that LPS plays in this protection-pathology orchestration is not just fascinating, but crucial to therapeutic design and drug safety.

LPS-Induced Mechanism of Action (TLR4-Related).2,3Fig.1 LPS-Induced TLR4-Mediated Signaling Pathways. 2,3

LPS Analysis Service

Our LPS analysis service is meticulously designed to address the diverse needs of researchers and industries. From vaccine development to drug safety assessments, we provide unparalleled support through advanced methodologies and customizable solutions.

Method Description Applications Advantages
SDS-PAGE Electrophoretic technique for profiling LPS structural components. Identifying molecular size, and glycan profiling. Fast, cost-effective, and reliable.
Mass Spectrometry Precision analysis of LPS molecular composition, focusing on lipid A and polysaccharides. Vaccine adjuvant research, drug development. High sensitivity, detailed molecular characterization.
ELISA Quantitative detection of LPS in biological and environmental samples. Endotoxin monitoring in manufacturing. High specificity, reproducible, and user-friendly.
LAL Assay Widely accepted method for endotoxin detection using Limulus Amebocyte Lysate. Regulatory safety testing for pharmaceuticals. Highly sensitive, gold-standard method.
Recombinant Factor C Assay Animal-free alternative to the LAL assay, ensuring ethical compliance. Pharmaceutical quality control. High specificity, ethical, and robust performance.
Biosensor Technology Advanced method for real-time monitoring of LPS interactions. High-throughput screening, immunological research. Scalable, precise, and innovative.

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Service Features

Comprehensive Molecular Profiling

Creative Biolabs provides in-depth molecular characterization of LPS, covering purity, structural insights, mechanistic understanding, and molecular species analysis. This enables researchers to link LPS structures to their biological activities, advancing studies in immunology and pharmaceutical development.

Integrated Immunological Insights

Our services combine LPS analysis with immunological assays like the Monocyte Activation Test, offering a complete view of endotoxin activity and its role in immune modulation and therapeutic innovation.

Customizable and Flexible Solutions

We adapt workflows, detection methods, and analysis strategies to meet diverse client needs. From biopharmaceuticals to industrial samples, our customizable services deliver reliable and precise outcomes tailored to specific research goals.

Real-Time Data and Diverse Sample Coverage

With real-time data tracking and the capability to analyze a wide range of LPS sources—including biological fluids, vaccines, and industrial products—our service ensures accuracy, transparency, and actionable insights for every project.

Applications

Biopharmaceutical Safety

LPS contamination in biologics can trigger adverse immune reactions. Our Endotoxin Detection Service ensures products meet safety standards, protecting patients and maintaining regulatory compliance.

Vaccine Development

LPS characterization supports the development of effective vaccines by aiding in antigen validation and adjuvant optimization. Our methods, including the Recombinant Factor C Assay, provide ethical and precise analysis.

Environmental and Industrial Monitoring

Advanced techniques like the Membrane Adsorption Assay are instrumental in detecting bacterial endotoxins in industrial processes and environmental samples.

Immunotherapy Research

Our service supports studies into LPS's immunomodulatory effects, paving the way for innovative therapies targeting inflammatory and autoimmune diseases.

With a team of professional and well-equipped experts, Creative Biolabs is dedicated to working with researchers around the world to meet customer requirements and ensure the quality of our LPS analysis service. For more detailed information, please feel free to contact us or directly send us an inquiry.

References

  1. Mohammad, Shireen, and Christoph Thiemermann. "Role of metabolic endotoxemia in systemic inflammation and potential interventions." Frontiers in immunology 11 (2021): 594150.
  2. Zamyatina, Alla, and Holger Heine. "Lipopolysaccharide recognition in the crossroads of TLR4 and caspase-4/11 mediated inflammatory pathways." Frontiers in Immunology 11 (2020): 585146.
  3. Distributed under Open Access license CC BY 4.0, without modification.

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