PathFinder™ CTLA4 : B7-2[Biotinylated] Inhibitor Screening Assay Kit (CAT#: IOK-02P044)

CTLA4: B7-1 TR-FRET assay aims to measure the inhibitory effect on the binding of CTLA4 to B7-1 in a uniform 384 response format. This FRET-based assay does not require time-consuming washing steps, so it is particularly suitable for high-throughput screening applications. The measurement process is simple and straightforward. The samples containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor and inhibitor were incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

The CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of CTLA4 binding to B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

This kit is useful for screening for inhibitors of CTLA4 binding to B7-2.

Cytotoxic T-lymphocyte-associated protein 4 CD152, T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, B72