De Novo Protein Sequencing Services
While antibody de novo sequencing holds significance in the biomedical field, there are numerous other types of protein samples beyond antibodies that require de novo sequencing. Through de novo sequencing techniques, we can obtain accurate amino acid sequences of these proteins, enabling a deeper understanding of their structures and functions, and thereby providing robust support for biomedical research and drug development. Creative Biolabs offers de novo protein sequencing services, powered by our propriety DASS (Database Assisted Shotgun Sequencing) technology to meet the diverse protein research needs of our clients, driving innovation and advancement in the field of biomedical science.
Fig.1 Peptide coverage and contigs of CD147.1
The Necessity of Protein Sequencing
De novo protein sequencing stands as an innovative technique devoid of reliance on any preexisting sequence or information present within protein databases. This methodology directly ascertains the amino acid sequence of a protein, thereby furnishing valuable insights into its underlying structure and functional attributes.
Understanding Protein Structure and Function
The sequence of a protein dictates its structure and function. De novo protein sequencing enables the precise determination of amino acid sequences, helping scientists comprehend the structure and function of proteins. This reveals their roles and biological functions within cells.
Protein Interaction Studies
Numerous biological processes involve interactions between proteins. De novo protein sequencing can provide foundational information for studying protein interactions, aiding in understanding binding partners and mechanisms of interaction.
Biopharmaceutical Development
Beyond antibodies, many other types of biopharmaceuticals encompass proteins, such as fusion proteins, recombinant proteins, enzymes, and hormones. Protein DNseq is crucial for biopharmaceutical research and quality control, ensuring the accuracy and effectiveness of proteins.
Proteins Requiring De novo Sequencing
Protein Family Members
De novo sequencing contributes to the precise discernment of resemblant family constituents which may elude differentiation via conventional database inquiries. This facilitates an enhanced comprehension of the distinctive functional variances inherent in biological processes.
Unknown Proteins
Despite the abundance of sequence information for known proteins in biological databases, there are still numerous unknown proteins. De novo sequencing is a crucial method for discovering and deciphering unknown proteins.
Biomarker Proteins
In many diseases, specific proteins produced by cells and other substances can serve as biomarkers. De novo sequencing can identify and characterize these biomarker proteins, aiding in early disease diagnosis and treatment.
Recombinant Proteins
Recombinant proteins used in biopharmaceuticals also require de novo sequencing to ensure their integrity and accuracy.
Mutated Proteins
Certain proteins may undergo mutations that can impact their function and stability. De novo sequencing can uncover these mutations and facilitate in-depth studies.
Protein Modifications
Many proteins undergo post-translational modifications such as phosphorylation and methylation. De novo sequencing helps determine these modification sites, furthering research into how protein modifications regulate biological functions.
De novo Protein sequencing Service Workflow at Creative Biolabs
Sample Preparation
Enzymatic Digestion and Fragmentation
Mass Spectrometry Analysis
Data Acquisition and Interpretation
Peptide Assembly and Validation
Bioinformatics Analysis
Report Delivery
By harnessing the advantages of high-resolution mass spectrometers and advanced algorithms throughout the entire de novo protein sequencing process, Creative Biolabs is equipped to provide clients with high-quality protein sequence information. If you are seeking a reliable partner for de novo protein sequencing, please do not hesitate to contact us for more information on service details and customized solutions.
Reference
- Mai, ZB.; et al. Highly robust de novo full-length protein sequencing. Analytical Chemistry. 2022, 94(8):3467-3475.