Other Mass Spectrometry (MS)-based Services
The delineation of protein sequences carries profound significance in the realms of research, pharmaceutical advancement, and therapeutic interventions. In the pursuit of enhanced precision, Creative Biolabs has pioneered the implementation of Database Assisted Shotgun Sequencing (DASS), thereby furnishing a platform for augmented accuracy and dependability in the identification of protein sequences through mass spectrometry (MS) techniques. This commitment guarantees our clientele access to a realm of superior data quality and comprehensive insights.
What is DASS?
Database Assisted Shotgun Sequencing (DASS) represents a pioneering advancement grounded in mass spectrometry (MS) for the intricate task of protein sequencing and comprehensive analysis. Operating at the forefront of technological sophistication, this methodology harnesses the potential of a mass spectrometer to extract intricate mass spectral information from protein specimens. Thereafter, through the intricate orchestration of cutting-edge data processing algorithms in-house, accentuated by purpose-built MS software, this extracted mass spectral data metamorphoses into a rich tapestry of amino acid sequence information, meticulously elucidating the protein's structural essence.
Advantages of DASS
Comprehensive Sequence Coverage
DASS achieves full sequence coverage of antibodies, ensuring accurate determination of all amino acid positions, especially in the variable regions of antibodies. Each position in these regions is covered at least 4 times, enhancing the precision of sequencing results.
High-Resolution Mass Spectral Data
DASS utilizes MS to obtain high-resolution peptide information, accurately analyzing various features of antibodies, and overcoming resolution limitations of traditional sequencing methods.
Identification of Crucial Information
DASS, in conjunction with MS data, can precisely identify key information such as mutations, post-translational modifications, glycosylation, etc., contributing to a deeper understanding of antibody structure and function.
Overcoming Amino Acid Discrimination Challenges
DASS can differentiate between amino acids with similar molecular weights, overcoming the limitations of traditional techniques in identifying indistinguishable amino acids.
Wide Applicability
DASS is suitable for antibodies from diverse species sources including mouse, rat, chicken, rabbit, sheep, etc. It's also applicable to sequencing various antibody subtypes and forms such as IgM, fluorescent-conjugated antibodies, immobilized antibodies, and more.
Support of Data Processing Algorithms
DASS employs advanced data processing algorithms and collaborates with MS software to achieve accurate identification and interpretation of antibody sequences.
Applications of DASS
1Antibody Sequencing
DASS achieves full sequence coverage, addressing limitations of traditional sequencing methods, including challenges in amino acid discrimination and identification of specific amino acids. This enables precise identification of antibody structure and function.
2Antibody Production and Engineering
DASS can be used for customized antibody production and engineering, including sequencing antibodies from different species sources, isotypes, and forms, providing comprehensive support for antibody research and applications.
3Protein Drug Development
DASS accurately identifies key information such as mutations and modifications in protein drugs, optimizing design and performance. This provides support for protein drug development.
4Protein-Protein Interaction Studies
Through advanced algorithms and scientific expertise, DASS enables more accurate identification and quantification of protein-protein interactions, assisting researchers in gaining deeper insights into the mechanisms of protein interactions.
Comprehensive and High-Quality Protein Analysis Services Based on DASS at Creative Biolabs
Leveraging the DASS algorithm and the expertise of our scientists, Creative Biolabs offers the following specific analysis services:
As recognized for innovation and leadership in the industry, Creative Biolabs is committed to delivering top-notch DASS services to worldwide clients. With our extensive sequencing experience, professional team of scientists, and advanced instrumentation, we excel in providing exceptional services. Whether it's achieving complete sequence coverage of antibodies, sequencing variable regions, or conducting an in-depth analysis of specific protein structures, Creative Biolabs delivers precise, comprehensive, and high-quality results. If you're seeking cutting-edge protein sequencing solutions, please don't hesitate to contact us.