Manual Sequence Curation

Creative Biolabs employs manual sequence curation in protein sequencing to ensure the provided sequencing results possess high quality, reliability, and trustworthiness.

Possible Errors in Automated Sequencing

Despite the continuous advancement and increased precision of automated sequencing technologies, various errors can occur, mainly when dealing with complex samples. These errors might impact the accuracy and reliability of protein sequencing, especially in cases involving intricate samples or samples with unique structures.

Isobaric Exchanges

When a protein contains isobaric exchanges, where two amino acids share the same molecular weight but have different chemical properties (e.g., N=GG or Q=GA), automated algorithms might fail to accurately identify such exchanges, leading to incorrect sequences.

Residue Inversion

In protein sequences, the order of amino acids could be mistakenly inverted, for instance, GP being interpreted as PG. Such instances can result in erroneous sequence analysis outcomes.

Fig.1. Flipped positions in a peptide mapping experiment. (Creative Biolabs Original)
The exchange has no impact on coverage, although the sequence might be wrong.

The Necessity of Manual Sequence Curation

Due to the presence of the aforementioned errors, the introduction of manual sequence curation becomes imperative.

Accuracy of Complex Samples

For complex protein samples containing isotopic exchanges, residue inversions, or other intricate variations, automated sequencing algorithms might struggle to accurately identify them. Manual sequence curation can address these complex situations and ensure sequencing results' accuracy.

Avoidance of Erroneous Results

Automated algorithms might produce erroneous results due to data noise, weakened signals, or other technical limitations. Through manual curation by experienced scientists, these errors can be identified and corrected, ensuring customers receive accurate and reliable sequencing outcomes.

Enhancement of Data Quality

Manual sequence curation can eliminate potential errors, thereby elevating data quality. High-quality sequencing data forms the foundation for biological research and applications, assisting customers in making precise scientific inferences.

Analysis of Special Structures & Regions

In some cases, specific structures and regions of a protein could pose challenges, such as low region overlap or inverted sequences. Manual curation can focus on these critical areas, guaranteeing their sequences are correctly identified and validated.

Protein Sequencing Services Enhanced by Manual Sequence Curation at Creative Biolabs

Building upon exceptional automated technology, Creative Biolabs intelligently incorporates manual sequence curation to provide high-quality protein sequencing services. This offers customers an outstanding and reliable sequencing solution.

Fig.2. Protein sequencing services enhanced by manual sequence curation. (Creative Biolabs Original)

De Novo Antibody Sequencing

De Novo Protein Sequencing

Other MS-based Services

By presenting manually curated spectral data in reports, customers can witness evidence supporting the results, enhancing credibility and transparency. If you're seeking a trustworthy protein sequencing service provider, please do not hesitate to contact us. Whether your research project involves complex samples, unique structures, variations, or even stringent requirements, Creative Biolabs can tailor solutions to your needs. Our experienced scientists will listen to your requirements and offer professional advice and support.

For Research Use Only | Not For Clinical Use

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