ALS is a severe neurodegenerative condition characterized by loss of motor neurons in the spinal cord and brain. This disease cell model has a L144FVX mutation in SOD1 gene. Generating pluripotent stem cells from fibroblasts, then differentiate into motor neurons . This disease cell model provides a platform to explore disease mechanisms in ALS.
Specifications | |
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Organ System | Central Nervous System |
Disease | Amyotrophic Lateral Sclerosis (ALS) |
Target Gene | SOD1 |
Gene Function | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. |
Mutation | L144FVX |
Phenotype | Loss of large motor neurons in the anterior horns associated with the frequent appearance of cord-like swollen, partially SOD 1- and ubiquitin-immunopositive axons. |
Source | Fibroblasts |
Cellular Assays | Immunocytochemistry, Patch-clamp experiments, Real-time PCR and sequence analysis, Western blotting and ELISA |
For Research Use Only. Not For Clinical Use.