Mesenchymal stem cells (MSC) have received significant attention in recent years with regard to their suitability for use in cellular therapies due to their high growth potential and multipotential differentiation capacity. As a trusted research partner in the field of biotherapeutics, Creative Biolabs provides cutting-edge cell therapy research services covering every stage of MSC development, including isolation, expansion, characterization, and differentiation.
Therapeutic Potential of MSCs
MSCs are a rare heterogeneous subset of pluripotent stem cells that are defined by their ability to undergo self-renewal and differentiation into cell types of mesodermal origin, including osteocytes, chondrocytes, and adipocytes. MSCs have been recognized as attractive therapeutic candidates for the treatment of various diseases (e.g., autoimmune, vascular, and degenerative conditions) due to their unique features. Firstly, MSCs are capable of differentiating into non-mesenchymal lineages, including neural cells, endothelial cells, astrocytes, cardiomyocytes, etc. The multipotency of MSCs has shown promise in the fields of regenerative medicine and tissue repair. Secondly, MSCs have been found to provide therapeutic benefit through the secretion of soluble factors to induce an immunomodulatory environment. Thirdly, MSCs have the capacity to migrate toward sites of injury and tumor microenvironments, thus allowing them to serve as delivery vehicles for targeted therapy. In addition, MSCs are widely accessible and can be expanded ex vivo conveniently with different protocols.
Fig.1 Clinical applications of MSCs.
Identification, Verification & Characterization of MSCs
Minimal criteria of multipotent MSCs proposed by the International Society for Cellular Therapy (ISCT) include adherence to plastic, specific surface antigen expression (CD73+, CD90+, CD105+, CD34-, CD45-, CD11b-, CD14-, CD19-, CD79a-, HLA-, DR-), as well as multipotent differential potential under the standard in vitro differentiation conditions. The publication of this criteria offers standards by which MSCs could be functionally and phenotypically defined.
Methods for MSC isolation, processing, characterization, expansion, and differentiation are critical because they could affect the MSC quality, functionality, and plasticity. Here, at Creative Biolabs, we are proud to provide comprehensive MSC isolation, expansion, characterization, and differentiation services.
Creative Biolabs is capable of providing MSCs obtained from different sources and tissues, including but not limited to bone marrow, umbilical cord blood and stroma, placenta, adipose tissue, as well as multiple dental tissues. Thereafter, MSCs are expanded in different cultures. We have established the optimal MSC isolation and expansion methods, as well as the best performing medium to provide our clients with high-quality MSCs ideally suited for different cellular applications.
Followed by the isolation and expansion from reliable sources, MSCs are required to be identified and characterized. To characterize and assess the quality of MSCs, Creative Biolabs can offer services including immunophenotyping analysis, karyotype analysis, short tandem repeat analysis, as well as evaluation of differentiation potential and immune modulation function.
The capabilities of MSC to differentiate into both mesenchymal and non-mesenchymal tissues have made them attractive candidates for biological cell-based tissue repair approaches. As a result, the monitoring and controlling of MSC differentiation are crucial. Creative Biolabs applies optimized media and supplements to efficiently drive the differentiation of MSCs to adipocytes, osteocytes, and chondrocytes.
Fig.2 Schematic diagram of the characteristics of MSCs. (Vedenko, 2014)
Long-term viability, multipotent potential, integrity, and uniformity of your cells are ensured
Amenable to the research of different cellular applications and assay endpoints
Adequate cell populations with the desired functionality and phenotype
Optimal culture insurance by ongoing technical support and a range of tailored media
Customized services and individualized support to suit specific research requirements
With the help of our well-established technologies and years of experience, Creative Biolabs is a well-recognized partner of choice to aid your cell therapy research. By providing either individual service modules or a fully comprehensive service to fulfill your requirements. Please don't hesitate to contact us for more information.
Vedenko, A.; et al. Mesenchymal Stem Cells: Characterization, Properties and Therapeutic Potential. 2014.