Based on the leading-edge platform and profound knowledge in cell immortalization, the specialized and dedicated team at Creative Biolabs provides world-class solutions for B cell immortalization and helps you obtain the desired results. Our state-of-the-art technologies and highly qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the service lifecycle.
Since it was recognized that the Epstein Barr Virus (EBV) could be used in vitro to infect human B lymphocytes and produce immortalized polyclonal B cell lines in culture, this technique has been used with great effect to facilitate immunological and molecular studies. EBV belongs to the herpesvirus family and is the main agent that causes human mononucleosis. The EBV infects B lymphocytes and immortalizes and transforms the cells into long-term growing EBV-bearing lymphoblastoid cell lines (LCLs), which consistently express the viruses.
The EBV-based immortalization technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As a result, EBV immortalized B cell lines are extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA, expression RNA, and protein and system biology analysis. In addition, these cell lines can be used as a culture model for various studies, including functional and serological studies, molecular and genetic research, large-scale drug library screening, vaccine study, and high throughput biological studies.
B cell immortalization has important applications in stem cell research, particularly in the generation of induced pluripotent stem cells (iPSCs). B cell immortalization can provide a virtually infinite supply of cells that can be used for reprogramming into iPSCs. In addition, B cells can be easily obtained from peripheral blood or other tissues, making them a readily available source of cells for iPSC generation.
EBV immortalization of B cell line is considered as the method of choice for generating LCLs. Immortalized cells are important models for studies in cell biology, cellular metabolism, and differentiation; and can be used for research in cancer biology, immunology, neurology, hematology, and more. With over a decade of experience and state-of-the-art platforms, Creative Biolabs provides highly custom solutions to effectively support your B cell immortalization projects. Our outstanding QC of immortalized cell lines characterized according to your requirements includes mRNA expression, protein expression, or functional assays.
As an experienced expert in cell immortalization, Creative Biolabs provides custom-specific research and solutions. If you have any special needs in constructing an immortalized cell line as an iPSC research method, please contact us for this special service. Let us know what you need, and we will accommodate you. We look forward to working with you in the future.
For Research Use Only. Not For Clinical Use.
