Creative Biolabs is gladly to show a series of De Novo Antibody Sequencing services, which were developed based on its next generation antibody sequencing platform with the “Database Assisted Shotgun Sequencing” (DASS) technology. All species, isotypes and allotypes of antibodies can be sequenced with 100% coverage.
De Novo Chicken IgYs Sequencing Service
Creative Biolabs provides De Novo Chicken IgYs Sequencing Service based on its DASS technology. Chicken Y antibodies are the predominant serum immunoglobulins in birds, reptiles and amphibians. In the female, transfer of IgY from serum to egg yolk gives passive immunity to embryos and neonates, which is similar to placental IgG transfer in mammals that also gives passive immunity to the fetus. A major difference between IgY and IgG is the inability of Y antibodies in binding to bacterial Fc receptors, such as the staphylococcal protein A and streptococcal protein G.
There are several advantages associated with the development and usage of chicken IgYs including better immune responsiveness to mammalian antigens, higher affinity with persistent titer, non-invasive collection, simple and low cost isolation process, large yield and scalable production.
De Novo Human/Humanized Antibody Sequencing Service
The generation of human or humanized antibodies to human antigens is of particular interest for the treatment of a variety of diseases. Creative Biolabs is pleased to present world-leading De Novo Human/Humanized Antibody Sequencing service. Scientists from Creative Biolabs has developed De Novo antibody sequencing technology— Database Assisted Shotgun Sequencing (DASS), which is an analytical method that derives an antibody’s amino acid sequence from its tandem mass spectrum (MS/MS) without the assistance of a sequence database. It is quite different from another popular peptide identification approach– “database search”, which searches in a given database to find the target peptide.
Humanized antibodies are antibodies from non-human species whose protein sequences have been modified to increase their similarity to antibody variants produced naturally in humans. Humanization can be necessary when the process of developing a specific antibody involves generation in a non-human immune system. Humanized antibodies are distinct from chimeric antibodies. Although the creation of an antibody chimera is normally undertaken to achieve a more human-like antibody, simple chimeras of this type are not usually referred to as humanized. Rather, the protein sequence of a humanized antibody is essentially identical to that of a human variant, despite the CDRs responsible for the ability of the antibody to bind to its target antigen.
De Novo Rodent Antibody Sequencing Service
With the developed “Database Assisted Shotgun Sequencing” (DASS) technology, Creative Biolabs also provides rodent antibody sequencing service. Its De Novo rodent antibody sequencing utilizes computational approaches to deduce the sequence or partial sequence of rat antibodies directly from the experimental MS/MS spectra. Usually 20-70 different MS/MS spectra are generated for each amino acid (AA) position of the antibody. This insures 100% coverage and accuracy of the whole antibody in the meanwhile. Creative Biolabs has database for rodent as it has extracted all sequences from IMGT and NCBI. Its algorithm does well in assembling overlapping peptides puzzle with imperfect database.