Short Decsription
The kit is designed for in vitro quantitative measurement of Goat VTCN1 in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.
Description
This ELISA kit is a solid phase ELISA designed for quantitative determination of CostimulatorymoleculesB7H4 (B7-H4).
Applications
ELISA
Application Notes
The supplier is only responsible for the kit itself, but not for the samples consumed during the assay.
Please predict the concentration before assaying.
If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits, some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
Fresh samples without long time storage are recommended for the test.
Comment
It is recommended that all standards, controls and samples be run in duplicate.
The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
Microtiter plates should be allowed - come - room temperature before opening the foil bags.
Samples should be collected in pyrogen/endotoxin-free tubes.
Samples should be frozen if not analyzed shortly after collection.
When possible, avoid use of badly hemolyzed or lipemic serum.
Read absorbance immediately after adding the stop solution.
Because TMB is light sensitive, avoid prolonged exposure - light. Also avoid contact between TMB and metal, otherwise color may develop.
Target
VTCN1
Reactivity
Goat
Detection Method
Colorimetric
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

For Research Use Only | Not For Clinical Use

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