Description
This kit has been developed to isolate highly purified CD4+ T cells from human peripheral blood mononuclear cells (PBMCs) by immunomagnetic negtive selection. Isolated cells are immediately available for downstream applications such as flow cytometry, phenotypic characterization, culture, or cell-based experiments.
Features
Fast and easy workflow takes only 30 minutes;
No magnet, no column, no harsh chemicals.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and deplete.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8, CD11b, CD14, CD16, CD19, CD36, CD56, CD123, CD235a, and TCR-γδ.
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research