Components
Coating antibodies; Biotinylated detection antibodies; Streptavidin-Horseradish Peroxidase; AEC coloring system; Blocking stock solution; Dilution buffer R; Tween-20; PVDF ELISpot plates with lids and cover slips;
Assay Procedure
See manual for details.
Assay Measures
T cell ELISpot assay is used to monitor cellular immune responses in vitro at the single-cell level. This method can accurately detect secreted proteins, such as cytokines, that T cells secrete in response to antigen release.
Storage
Depends on the product components.
Storage Comment
Store protected from light.
Restrictions
For research use only. Not for use in diagnostic procedures.