Short Decsription
PD-1 [Biotinylation]: The PD-L1 homogeneous assay kit is designed to measure; the inhibitory effect of PD-1 and PD-L1 binding. PD-1 [Biotinylation]: PD-L1 homogeneous detection kit; using convenient AlphaLISA® format, with purified biotinylated PD-1, PD-L1 with FLAG tag; analysis buffer for total 384 reactions. With this kit, only three simple steps are required; a microtiter plate is required. First, the sample contains biotinylated PD-1 and its inhibitors. Choose to incubate with FLAG-labeled PD-L1 for 60 minutes. Next, the acceptor beads; first add the donor beads, and then read the Alpha count.
Description
The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit is designed to measure; the inhibition of PD-1 binding to PD-L1. The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit; comes in a convenient AlphaLISA庐 format with purified biotinylated PD-1, FLAG-tagged PD-L1,; and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a; microtiter plate are required. First, a sample containing biotinylated PD-1 and an inhibitor of; choice is incubated with the FLAG-tagged PD-L1 for 60 minutes. Next, acceptor beads are; added, then donor beads, followed by reading the Alpha-counts.
Applications
Useful for screening for inhibitors of PD-1 binding to PD-L1
Target
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC