Cow EDNRB ELISA Kit, Lot 21AO-983 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P894)

The kit is designed for in vitro quantitative measurement of Cow EDNRB in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.

This ELISA kit is a solid phase ELISA designed for quantitative determination.

ELISA

The supplier is only responsible for the kit itself, but not for the samples consumed during the assay.
Please predict the concentration before assaying.
If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits, some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
Fresh samples without long time storage are recommended for the test.

It is recommended that all standards, controls and samples be run in duplicate.
The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
Microtiter plates should be allowed - come - room temperature before opening the foil bags.
Samples should be collected in pyrogen/endotoxin-free tubes.
Samples should be frozen if not analyzed shortly after collection.
When possible, avoid use of badly hemolyzed or lipemic serum.
Read absorbance immediately after adding the stop solution.
Because TMB is light sensitive, avoid prolonged exposure - light. Also avoid contact between TMB and metal, otherwise color may develop.

EDNRB

Cow

Colorimetric

Competition ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate