Description
This kit has been developed to isolate highly purified mouse CD3+ T cells from murine splenocytes. The CD3 Fab is a recombinantly expressed low affinity Fab fragment. The CD3 Fab-Strep binds to the matrix of Strep-Agarose columns. Therefore, CD3+ target cells can adhere to the matrix. Finally, the addition of biotin causes the elution of the target cells from the matrix, and Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
Highly pure cells;
Label-free cells
Applications
Cell Isolation
Application Notes
Desired cells are labeled with low affinity CD3 Fab.
Comment
This kit is available for processing 1 x 10^8 splenocytes.
Target
CD3+ Cell
Method Type
Positive selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Murine splenocytes

For Research Use Only | Not For Clinical Use

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