PharmaOnco™ Jurkat-TCR KO//B2M KO-NFAT Luc Reporter Cell Line, Single Clone (CAT#: ITS-0822-HMM210)

Creative Biolabs offers Jurkat-TCR KO//B2M KO-NFAT Luc reporter cell line which the TCR and B2M gene were knocked out in NFAT Luc reporter Jurkat cells.

Jurkat-TCR KO//B2M KO-NFAT Luc reporter cell line is a cell line derived from Jurkat cells, which are a human T lymphocyte cell line. The cell line has been genetically modified to knockout the T cell receptor (TCR) and beta-2 microglobulin (B2M) genes. Additionally, the cell line contains a luciferase reporter gene under the control of the NFAT (nuclear factor of activated T cells) promoter, allowing for the monitoring of NFAT activation. This cell line is a valuable tool for studying immune responses and T cell signaling pathways, as the absence of TCR and B2M prevents T cell activation through the TCR complex. The NFAT luciferase reporter provides a readout of NFAT activation, which is a key transcription factor involved in T cell activation and cytokine production.

Loss of gene function;
Stability and Specificity;
Reliability and reproducibility;

Functional assay

TCR//B2M

Human

Jurkat

The TCR gene, or T-cell receptor gene, is a gene responsible for encoding the protein receptors on the surface of T cells. T cells are a type of white blood cell that play a central role in the immune system, recognizing and responding to foreign antigens such as viruses, bacteria, and cancer cells. There are two main types of TCR genes, alpha and beta, which combine to form the T-cell receptor complex. These receptors are highly diverse and can recognize a wide variety of antigens, allowing T cells to mount specific immune responses to different threats. Mutations or dysregulation of the TCR gene can lead to immune system dysfunction, resulting in conditions such as autoimmune diseases, immunodeficiency disorders, and cancer. Studying the TCR gene and its role in immune function is important for understanding and developing treatments for these conditions.
The B2M gene encodes the Beta-2 microglobulin protein, which plays a role in the immune system by forming part of the major histocompatibility complex (MHC) class I molecule. This molecule is important for presenting antigens to T cells, allowing the immune system to recognize and destroy infected or abnormal cells. Mutations in the B2M gene can lead to various immune system disorders, including autoimmune diseases and immunodeficiency. Additionally, elevated levels of Beta-2 microglobulin in the blood have been associated with certain types of cancer, such as multiple myeloma and lymphoma.